SYLLABUS

Module

Course Description Hrs CO.No.
1.0 BASICS OF ANIMAL CELL CULTURE 11  
1.1 Structure of animal cell 1 1
1.2 History of animal cell culture 1 1
1.3 Different types of cell culture media, growth supplements, serum free media, balanced salt solution 1 2
1.4 Culture of different tissues and its application 1 2
1.5 Infra structure requirements 1 2
1.6 Conditions required for culturing animal cells, Behavior of cells in culture conditions, division, their growth pattern, Estimation of cell number 1 2
1.7 Culture of mammalian cells, tissues and organs, primary culture, secondary culture, Continuous cell lines, suspension cultures 1 2
1.8 Stem cells 1 2
1.9 Cryopreservation 1 2
1.10 Common cell culture contaminants 1 2
1.11 Commercial scale production of animal cells. 1 2
2.0 IN VITRO APPLICATION OF ANIMAL CELL CULTURE 6  
2.1 Application of animal cell culture for in vitro testing of drugs, testing of toxicity of Environmental pollutants in cell culture 2 3
2.2 Application of cell culture technology in production Of human and animal viral vaccines and pharmaceutical protein 2 3
2.3 Conventional methods of animal vaccine production, recombinant approaches to vaccine production 2 3
2.4 Commercial scale production of diagnostic antigens and antisera 2 3
3.0 ADVANCED APPLICATION OF CELL CULTURES 17  
3.1 Structure of sperms and ovum 1 4
3.2 Cryopreservation of sperms and ova of livestock 1 4
3.3 Artificial insemination, Super ovulation, in vitro fertilization 1 5
3.4 Culture of embryos, Cryopreservation of embroys 1 5
3.5 Embryo transfer, embryo -splitting, embryo sexing 1 5
3.6 In utero testing of foetus for genetic defects 1 5
3.7 Animal cloning basic concept, cloning from embryonic cells and adult cells 1 6
3.8 Cloning of different animals, Cloning for conservation of endangered species 1 6
3.9 Ethical, social and moral issues related to cloning 1 7
3.10 Transgenic manipulation of animal embryo, Animal viral vectors 1 8
3.11 Different application of transgenic animal technology 1 8
3.12 Transgenic animal production and application in expression of therapeutic proteins, biopharming. 1 8
3.13 Gene knock out technology and animal models for genetic disorders. 1 9
3.14 Gene therapy, somatic cell therapy, germline therapy,gene augmentation therapy, gene replacement therapy 1 9
3.15 Candidate diseases for Gene therapy 1 9
3.16 Methods of gene transfer, vectors used 1 9
3.17 Initial trials and observations, Current status of Gene therapy 1 9
4.0 BIO-NANOTECHNOLOGY 20  
4.1 Introduction to Bio-macromolecules 10  
4.1.1 The modern concepts to describe the conformation and dynamics of biological macromolecules 1 10
4.1.2 Scattering technique 2 10
4..1.3 Micromanipulation technique 1 10
4.1.4 Drug delivery applications 2 10
4.1.5 Cellular engineering: signal transduction in biological systems, feedback, control signalling pathways, cell-cell interactions etc 4 10
4.1.6 Effects of physical, chemical and electrical stimuli on cell function 1 10
4.2 Biomaterials and bioresponse 3  
4.2.1 Chemical, physical and biological properties of biomaterials and bioresponse 1 11
4.2.2 Biosynthesis, and properties of natural materials (proteins, DNA, and polysaccharides structureproperty relationships in polymeric materials ( synthetic polymers and structural proteins) 1 11
4.2.3 Aerosol, properties, ,application and dynamics Statistical Mechanics in Biological Systems 1 11
4.3 Nanoparticles: Preparation and Application 4  
4.3.1 Characteristics of nanoparticles: Preparation and characterization of nanoparticles 1 12
4.3.2 Biosynthesisofnanoparticles,Nanoparticularcarri ersystems,Micro-andNano-fluidics,Drug and gene delivery system, Microfabrication, Biosensors, Chip technologies, Nano-imaging 2 12
4.3.3 Metabolic engineering and Gene therapy 1 12

Text Books for Reference

 Gordon I. 2005. Reproductive Techniques in Farm Animals. CABI.

 Levine MM, Kaper JB, Rappuoli R, Liu MA, Good MF. 2004. New Generation Vaccines. 3rd Ed. Informa Healthcare.

 Lincoln PJ & Thomson J. 1998. Forensic DNA Profiling Protocols. Humana Press.

Text Books for Enrichment

 Portner R. 2007. Animal Cell Biotechnology. Humana Press.

 Nalwa HS. 2005. Handbook of Nanostructured Biomaterials and Their Applications in Nanobiotechnology. American Scientific Publ.

 Niemeyer CM & Mirkin CA. 2005. Nanobiotechnology. Wiley