Syllabus

Module Course Description Hrs   CO.No.
1.0 Atomic structure 5  
1.1 Introduction to atom and atomic structure: Brief history of discovery of subatomic particles and atomic structure 1 1
1.2 Rutherford Atomic Model, Bohr atom model, concepts of atomic radius and energy value. 1 1
1.3 Rydburg‟s constant, Bohr-sommerfeld model 1 1
1.4 Vector atom model, Quantization of energy levels, quantum numbers. 1 1
1.5 Pauli‟s exclusion principle, Selection rules. 1 1
2.0 Spectroscopy 20  
2.1 Electromagnetic spectrum: Definition, application of each region of EM spectrum for spectroscopy. 1 2
2.2 Introduction to molecular energy levels: absorption, excitation and emission 2 2
2.3 Electronic spectroscopy: UV-Visible spectroscopy: Principle, construction and working of spectrophotometer 2 3
2.4 colorimeter; Applications of UV-Visible spectroscopy 1 3
2.5 Fluorometer: Principle, instrumentation and working 2 3
2.6 Applications Fluorometery to biomolecules (proteins, DNA, Hb, chlorophyll) 1 3
2.7 Rotational and vibrational spectroscopy: Energy levels of diatomic vibrating molecules 1 3
2.8 IR spectroscopy, principle, constructing and working of IR spectrometer 1 3
2.9 Application of IR spectroscopy to biomolecules 1 3
2.10 Raman Spectroscopy 1 3
2.11 Nuclear magnetic resonance spectrometer (NMR) 2 3
2.12 Atomic absorption spectroscopy (AAS) 1 3
2.13 XRD 1 3
2.14 Mass spectroscopy 2 3
3.0 Radioactivity 10  
3.1 Nucleus – properties. Nuclear forces. Nuclear models (liquid drop and shell model) 1 4
3.2 Radioactive nucleus. Nuclear radiation and their properties – alpha, beta and gamma. 2 4
3.3 Half-life – physical and biological 1 4
3.4 Handling and standardization of alpha, and beta emitting isotopes. 2 4
3.5 Radioimmunoassay 1 4
3.6 Radiopharmaceuticals and its uptake – dosimetry and detection Principle-construction and working of pen and batch dosimeter 1 4
3.7 GM counter 1 4
3.8 Scintillation counter (solid and liquid) 1 4
4.0 Biophysics and Bioinstrumentation 19  
4.1 Thermodynamics: Enthalpy, entropy, free energy 1 5
4.2 Gibb‟s free energy (G) and Helmholtz free energy (A) 1 5
4.3 Chemical potential, half cell potential. Redox potential 1 5
4.4 Structure and bioenergetics of mitochondria and chloroplast 1 5
4.5 Body temperature and its regulation.      1       5
4.6 Cell membrane: Organization of plasma membrane 1 6
4.7 Mass transport, diffusion, basics, passive and active transport 1 6
4.8 Membrane potential. Nernst equation. Passive electrical properties of cell (capacitance and resistance). Active electrical properties. 1 6
4.9 Electrical model (equivalent) of cell membrane. Depolarization, hyperpolarization of membrane (neuronal). Generation of active potential. 2 6
4.10 Types of biopotentials. Biopotential measuring instruments 1 6
4.11 Bio-instrumentation: Types of thermometers (clinical, thermocouple, bimetallic, platinum resistance, thermistorthermometers) 2 7
4.12 Principle construction, working and applications of instruments for analysis of biomolecules: pH meter 1 7
4.13 Electrophoresis 1 7
4.14 Centrifuge (RCF, sedimentation concept), different types of centrifuges 2 7
4.15 Construction, working and sample preparation of SEM 1 7
4.16 Construction, working and sample preparation of TEM and STEM 1 7

 

Text Books for Reference

 Principles of Physical Chemistry: Puri, Sharma and Pathania (Vishal Publishing Co., Jalandhar)

Nuclear Physics: an introduction: SB Patel (New Age International)

 Introduction to Atomic Spectra: HE White (Mc Graw Hill)

 Text Book of optics and atomic physics: P P Khandelwal (Himalaya publications)

 Biophysics: Cotrell (Eastern Economy Edition).

 Clinical Biophysics: Principles and Techniques: P Narayanan (Bhalani Publ., Mumbai). Biophysics: Pattabhi and Gautham.