Syllabus

Module Course Description Hrs CO.No
1.0 Spectral properties 6  
1.1 UV/Visible Spectral analysis of colouring pigments: Beta cyanin/ Anthocyanin/ Xanthine/ Lycopene and Curcumin 4 8
1.2 Colorimetric assays 2 8
2.0 Separation Techniques 6  
2.1 Chromatography (PC, TLC and Column) 4 8
2.2 GC & HPLC, HPTLC (Demonstration only) 2 8
3.0 Electrophoretic separation of protein 6  
3.1 Polyacrylamide gel electrophoresis 6 8

 

REFERENCE

 Jay Nadeau (2015). Introduction to Experimental Biophysics (Set): Textbook and Lab Manual by CRC Press

 Principles and Techniques of Biochemistry and Molecular Biology (2010). 7 th edition Edited by Keith Wilson, University of Hertfordshire, John Walker, University of Hertfordshire Cambridge University Press

 Alexander P, Lundgren HP: A Laboratory Manual of Analytical Methods of Protein Chemistry. Vols. 1–5, Pergamon Press, Oxford. 1966–69.

 Plummer DT (1987). An Introduction to Practical Biochemistry. 3rd ed., McGraw-Hill, London