| Module |
Course Description |
Hrs | CO.No. |
| 1.0 | PHYSICAL ASPECTS OF BIOCHEMISTRY | 10 | |
| 1.1 | Structure of water | 1 | 1 |
| 1.2 | Interactions in aqueous systems- covalent bond, hydrophobic interactions, ionic interactions. | 1 | 2 |
| 1.3 | Interactions in aqueous systems- hydrogen bond and van der Waals interactions. | 1 | 2 |
| 1.4 | Ionization of water, strong and weak acids and their dissociation. | 1 | 3 |
| 1.5 | Ionization of strong and weak bases and their dissociation. | 1 | 3 |
| 1.6 | Henderson-Hasselbalch equation with derivation. | 1 | 4 |
| 1.7 | Concepts of pH. | 1 | 4 |
| 1.8 | Concepts of Buffers | 1 | 4 |
| 1.9 | Buffers in biological systems – Phosphate buffer, Bicarbonate Buffer, Hemoglobin buffer. | 1 | 4 |
| 1.10 | Buffers in biological systems – Bicarbonate Buffer, Hemoglobin buffer | 1 | 4 |
| 2.0 | MEMBRANE BIOCHEMISTRY | 8 | |
| 2.1 | Fluid mosaic model of membrane. | 1 | 5 |
| 2.2 | Types of membrane proteins (peripheral, integral and amphitropic). | 1 | 6 |
| 2.3 | Solute transport across membranes (passive transport – simple diffusion and facilitated diffusion). | 1 | 7 |
| 2.4 | Solute transport across membranes (active transport – primary transport). | 1 | 7 |
| 2.5 | Solute transport across membranes (active transport – secondary transport – uniport, symport, antiport). | 1 | 7 |
| 2.6 | Osmosis. | 1 | 7 |
| 2.7 | Fundamental study of Donnan equilibrium. | 1 | 8 |
| 2.8 | Donnan equilibrium-application in biological system. | 1 | 8 |
| 3.0 | PLANT BIOCHEMISTRY | 8 | |
| 3.1 | Basic ideas of photosynthesis – Structure of chloroplast. | 1 | 9 |
| 3.2 | Photosynthetic pigments. Absorption and utilisation of light energy by photosynthetic pigments. | 1 | 10 |
| 3.3 | Red drop and Emerson‟s enhancement effect. | 1 | 10 |
| 3.4 | Photosystems. Light reaction – cyclic photophosphorylation and noncyclic photophosphorylation. | 1 | 10 |
| 3.5 | Dark reaction, fixation of CO2 and formation of carbohydrate. | 1 | 10 |
| 3.6 | C3 and C4 plants. | 1 | 10 |
| 3.7 | Crassulacean Acid Metabolism. | 1 | 10 |
| 3.8 | Biological nitrogen fixation. | 1 | 11 |
| 4.0 | TECHNIQUES IN BIOCHEMISTRY | 10 | |
| 4.1 | Spectrophotometry. | 1 | 12 |
| 4.2 | Colorimetry. | 1 | 12 |
| 4.3 | Chromatography – Paper, TLC, HPTLC | 1 | 12 |
| 4.4 | Chromatography – Gel Filtration, Affinity chromatography. | 1 | 12 |
| 4.5 | Electrophoresis – PAGE, AGE | 1 | 12 |
| 4.6 | Electrophoresis – PAGE, AGE. | 1 | 12 |
| 4.7 | Blotting Techniques – Western Blotting. | 1 | 12 |
| 4.8 | Blotting Techniques – Southern Blotting and Northern Blotting. | 1 | 12 |
| 4.9 | Introduction to proteomics. | 1 | 12 |
| 4.10 | Introduction to proteomics – MALDI – TOF MS. | 1 | 12 |
Text Books for Reference
Fundamentals of Biochemistry by J. L. Jain, Sunjay Jain and Nitin Jain, (2008) Publishers: S. Chand & Co Ltd ISBN: 81-219-2453-7 p: 230, 244, 269.
Lehninger Principles of Biochemistry by Nelson, D. L., Lehninger, A. L., & Cox, M. M. (2008) 5thEdition, ISBN: 978-0-230-22699-9, Publisher: W. H. Freeman and, Company, New York
Plant Biochemistry: P.M. Dey and J.B. Harborne. (Editors.) Harcourt Asia PTE Ltd. Academic Press. (Indian Edition, 2000).
Text Books for Enrichment
Biochemistry fifth edition by campbellfarrell (2006) Thomson Brooks cole Ltd p:34-54.
Biochemistry by J. M. Berg, J. L.Tymoczko, L. Stryer6th edition(2007 )W. H. Freemanand Company, New Yorkp: 510-555.
Plant Metabolism: H.D. Kumar and H.N. Singh. Affiliated East-West Press Pvt. Ltd., New Delhi, Madras, Hyderabad and Bangalore. (1993; 2nd edition).