Syllabus

Module

Course Description

Hrs CO.No.
1.0 PHYSICAL ASPECTS OF BIOCHEMISTRY 10  
1.1 Structure of water 1 1
1.2 Interactions in aqueous systems- covalent bond, hydrophobic interactions, ionic interactions. 1 2
1.3 Interactions in aqueous systems- hydrogen bond and van der Waals interactions. 1 2
1.4 Ionization of water, strong and weak acids and their dissociation. 1 3
1.5 Ionization of strong and weak bases and their dissociation. 1 3
1.6 Henderson-Hasselbalch equation with derivation. 1 4
1.7 Concepts of pH. 1 4
1.8 Concepts of Buffers 1 4
1.9 Buffers in biological systems – Phosphate buffer, Bicarbonate Buffer, Hemoglobin buffer. 1 4
1.10 Buffers in biological systems – Bicarbonate Buffer, Hemoglobin buffer 1 4
2.0 MEMBRANE BIOCHEMISTRY 8  
2.1 Fluid mosaic model of membrane. 1 5
2.2 Types of membrane proteins (peripheral, integral and amphitropic). 1 6
2.3 Solute transport across membranes (passive transport – simple diffusion and facilitated diffusion). 1 7
2.4 Solute transport across membranes (active transport – primary transport). 1 7
2.5 Solute transport across membranes (active transport – secondary transport – uniport, symport, antiport). 1 7
2.6 Osmosis. 1 7
2.7 Fundamental study of Donnan equilibrium. 1 8
2.8 Donnan equilibrium-application in biological system. 1 8
3.0 PLANT BIOCHEMISTRY 8  
3.1 Basic ideas of photosynthesis – Structure of chloroplast. 1 9
3.2 Photosynthetic pigments. Absorption and utilisation of light energy by photosynthetic pigments. 1 10
3.3 Red drop and Emerson‟s enhancement effect. 1  10
3.4 Photosystems. Light reaction – cyclic photophosphorylation and noncyclic photophosphorylation. 1 10 
3.5 Dark reaction, fixation of CO2 and formation of carbohydrate. 1 10 
3.6 C3 and C4 plants. 1 10 
3.7 Crassulacean Acid Metabolism. 1 10 
3.8 Biological nitrogen fixation. 1  11
 4.0  TECHNIQUES IN BIOCHEMISTRY  10  
4.1   Spectrophotometry.  1 12 
4.2   Colorimetry. 12 
4.3   Chromatography – Paper, TLC, HPTLC 12 
4.4   Chromatography – Gel Filtration, Affinity chromatography. 12
4.5   Electrophoresis – PAGE, AGE 12 
4.6   Electrophoresis – PAGE, AGE. 12 
4.7   Blotting Techniques – Western Blotting. 12
 4.8  Blotting Techniques – Southern Blotting and Northern Blotting. 12  
4.9   Introduction to proteomics. 12 
4.10   Introduction to proteomics – MALDI – TOF MS. 12 

Text Books for Reference

 Fundamentals of Biochemistry by J. L. Jain, Sunjay Jain and Nitin Jain, (2008) Publishers: S. Chand & Co Ltd ISBN: 81-219-2453-7 p: 230, 244, 269.

 Lehninger Principles of Biochemistry by Nelson, D. L., Lehninger, A. L., & Cox, M. M. (2008) 5thEdition, ISBN: 978-0-230-22699-9, Publisher: W. H. Freeman and, Company, New York

 Plant Biochemistry: P.M. Dey and J.B. Harborne. (Editors.) Harcourt Asia PTE Ltd. Academic Press. (Indian Edition, 2000).

Text Books for Enrichment

 Biochemistry fifth edition by campbellfarrell (2006) Thomson Brooks cole Ltd p:34-54.

 Biochemistry by J. M. Berg, J. L.Tymoczko, L. Stryer6th edition(2007 )W. H. Freemanand Company, New Yorkp: 510-555.

 Plant Metabolism: H.D. Kumar and H.N. Singh. Affiliated East-West Press Pvt. Ltd., New Delhi, Madras, Hyderabad and Bangalore. (1993; 2nd edition).