Syllabus

Module Course Description Hrs CO. No.
1.0 Preparation of solutions, buffers and determination of pH 8  
1.1 Molar solutions and Normal solutions 2 1
1.2 Dilution of Stock solutions 2 1
1.3 Preparation of buffers using the Henderson Hasselbalch equation 2 1
1.4 Determination of pH using pH meter (Demonstration) 2 2
2.0 Biochemical separation Techniques 20  
2.1 Chromatographic techniques (Any one to be performed) – Part 1 Separation of amino acids and simple sugars by Paper chromatography (Descending or ascending) Separation of amino acids and lipids by Thin Layer chromatography Separation of Plant pigments by Column/ Thin layer chromatography 5 2
2.2 Chromatographic techniques (Any one to be performed) – Part 2 Separation of amino acids and simple sugars by Paper chromatography (Descending or ascending) Separation of amino acids and lipids by Thin Layer chromatography Separation of Plant pigments by Column/ Thin layer chromatography 5 2
2.3 Chromatographic techniques (Any one to be performed) – Part 3 Separation of amino acids and simple sugars by Paper chromatography (Descending or ascending) Separation of amino acids and lipids by Thin Layer chromatography Separation of Plant pigments by Column/ Thin layer chromatography 5 2
2.4 Electrophoretic techniques (Demonstration) Analysis of proteins and nucleic acids (PAGE, AGE) 5 2
3.0 Colorimetry and Spectrophotometry techniques 8  
3.1 Verification of Beer Lambert‟s law – Part – 1 4 3
3.2 Verification of Beer Lambert‟s law – Part – 2 4 3

REFERENCE

 Standard Methods of Biochemical Analysis, S. K. Thimmaiah (ed), Kalyani Publishers, Ludhiana ISBN 81-7663-067-5, p 12 – 18

 Practical Biochemistry Principles and Techniques by Keith Wilson and John Walker 5th edition (2005), Cambridge University Press, p: 580-681

 Biophysical Chemistry Principles and Techniques by Upadhyay Nath Upadhyay, Himalaya publishing house (2002), p: 175-270, 344-421, 422-478.