Syllabus

 

Module

Course Description

Hrs CO.No.
1.0 ENZYMOLOGY 16  1
1.1 Classification of enzymes – six major classes of enzymes with one example each.  2  2
1.2  Elementary study of the factors affecting velocity of enzyme catalyzed reactions- effect of substrate concentration.  1
1.3  Elementary study of the factors affecting velocity of enzyme catalyzed reactions- effect of enzyme concentration.  1
1.4  Elementary study of the factors affecting velocity of enzyme catalyzed reactions- effect of temperature.  1
1.5  Elementary study of the factors affecting velocity of enzyme catalyzed reactions- effect of pH.  1
1.6  Michaelis-Menten equation. Km and its significance.  1
1.7  The Lineweaver-Burk plot.  2
1.8  Cofactors and coenzymes 3
1.9  Group specificity of enzyme with example. 4
1.10  Optical specificity of enzyme with example. 4
1.11  Geometrical specificity of enzyme with example. 4
1.12  Cofactor specificity of enzyme with example.  1 4
2.0 CARBOHYDRATE METABOLISM 16  
2.1 Glycolysis (with structure). 3 5
2.2 Fates of pyruvate – lactic acid fermentation. 1 5
2.3 Fates of pyruvate – alcohol fermentation. 1 5
2.4 Pyruvate dehydrogenase reaction. 1 5
2.5 Citric acid cycle (with structure). 3 5
2.6 Substrate level phosphorylation. 1 5
2.7 Electron transport chain. 2 5
2.8 Oxidative phosphorylation. 2 5
2.9 Glycogen metabolism – glycogenesis. 1 5
2.10 Glycogen metabolism-glycogenolysis. 1 5
3.0 PROTEIN METABOLISM 12  
3.1 Decarboxylation of aminoacids. 2 6
3.2 Deamination of aminoacids. 1 6
3.3 Transamination of aminoacids. 2 6
3.4 Urea Cycle 3 7
3.5 Glucogenic amino acids with examples. 2 8
3.6 Ketogenic amino acids with examples. 2 8
4.0 LIPID METABOLISM 10  
4.1 Fatty acid biosynthesis (with structure). 3 9
4.2 Oxidation of fatty acids – Fatty acid activation. 1 10
4.3 Oxidation of fatty acids – Carnitine shuttle. 1 10
4.4 β-Oxidation of fatty acids (with structure) – explain using palmitic acid and ATP yield. 2 10
4.5 Ketone bodies. 1 11
4.6 Cholesterol biosynthesis (without structure). 2 12

Text Books for Reference

 A Text Book of Biochemistry by E.S. West, W.R. Todd, H.S. Mason and J.T. van Bruggen, Oxford and IBH Publishing Co., New Delhi, 1974.

 Harper’s Biochemistry by Robert K. Murray , Daryl K. Granner, Peter A. Mayes and Victor W. Rodwell, Publisher: Appleton & Lange; 25th Revised edition (1 July 1999), ISBN-10: 0838536840, ISBN-13: 978-0838536841.

 Biochemistry Seventh Edition by Jeremy M. Berg, John L. Tymoczko and Lubert Stryer, 74 Publisher: W. H. Freeman; Seventh Edition edition (December 24, 2010).

Text Books for Enrichment

 Biochemistry by Donald Voet, Judith G. Voet, Publisher: John Wiley & Sons (2011), Fourth Edition, ISBN-10: 0071737073, ISBN-13: 978-0071737074.

 Fundamentals of Biochemistry by J. L. Jain, Sunjay Jain, Nithin Jain (2008), Publishers: S. Chand & Co Ltd ISBN: 81-219-2453-7.

 Lehninger, Principles of Biochemistry by Nelson, D. L., Lehninger, A. L., & Cox, M. M.(2008), 5thEdition, ISBN: 978-0-230-22699-9, Publisher: W. H. Freeman and Company, New York, p: 677-878.