Syllabus

Module

Course Description Hrs CO.No.
1.0 Extraction of enzymes 12  
1.1 Extraction of Acid phosphatase from Fresh Potato (Solanum tuberosum) 6 1
1.2 Extraction of β- amylase from Sweet potato (Ipomoea batatas) or Extraction of Urease from Jack bean (Canavalia ensiformis) 6 1
2.0 Enzyme Assay 24  
2.1 Assay of Acid phosphatase – Part 1 6 2
2.2 Assay of Acid phosphatase – Part 2 6 2
2.3 Assay of β- amylase or Urease – Part 1 6 2
2.4 Assay of β- amylase or Urease – Part 2 6 2

REFERENCE

 Experimental Biochemistry: A Student Companion, Beedu Sasidhar Rao & Vijay Deshpande (ed), I.K International Pvt. LTD, New Delhi ISBN 81-88237-41-8, p: 173-187

 Introductory Practical biochemistry, S. K. Sawhney & Randhir Singh (eds) Narosa Publishing House, New Delhi, ISBN 81-7319-302-9, p: 110 – 155

 Standard Methods of Biochemical Analysis, S. K. Thimmaiah (ed), Kalyani Publishers, Ludhiana ISBN 81-7663-067-5, p: 49- 181, 184 – 255