Botany Faculty

Dr. Hari N

Assistant Professor

Education: BSc., Kerala University, 1996
MSc., Kerala University, 1998
PhD., MG University, 2004

Area of Specialization: Plant Physiology, Plant Anatomy, Phytochemistry, Molecular Biology, Pteridology and Palynology

Email: hari.n@cmscollege.ac.in

Awards & Recognition:

  1. Day of Excellence:

“Award for Ph.D.”

On 29thJuly 2006 of Mahatma Gandhi

University, Kottayam.

    (No. E.B.X/Ph.   D/2006, dtd.14thJuly 2006).

  1. Certificate of Excellence:

“Bharat Jyoti Award”

    For Meritorious Services, Outstanding   

    Performance and Remarkable Role.

   At a seminar on Economic Growth & National

    Integration at New Delhi on 13th August 2016.

  1. 3. “Rev. Joseph Peet Award for

    Research Excellence 2017-2018”

C M S College Kottayam.

1. Anatomical profiling and palynological studies in various groups of pants
2. Plant physiology and Phytochemistry in various groups of plants
3. Seed technology and DNA - barcoding

B01 B01U- Methodology
B02B02U- General Informatics
B05 B07U- Plant Breeding
B05B08u- Evolution
B06B010U- Gymnosperms
PC – 3- Pteridology
PC- 5 – Gymnosperms & Evolution
PC -7 – Plant Anatomy & Angiosperm Systematics
Pc-9- Research Methodology
PC -10- Plant Breeding
PE-2- Genetic Engineering
PE-3- Genomics

Journal of Academia and Industrial Research (JAIR)
Volume 5, Issue 7th December 2016

Comparative Study on Foliar and Petiole Anatomy of the Genus Bruguiera L. in Mangrove Forest of Kerala

S. Surya* and N. Hari

Department of Botany, C.M.S. College, Kottayam, Kerala-686001, India suryaidukki@gmail.com*,+91 9400094355*, drhariaiyer@gmail.com; 08547591199

Abstract

Leaf and petiole anatomical profiling of three species of Bruguiera L. occurring in the mangrove forest ofKerala was investigated in this study. The study aims to search for the stable features marking out Bruguiera species. Anatomical characters in all three taxa showed similarities because of its generic closeness.Three species were distinguished based on the difference in the number of vascular bundles in midrib andpetiole, number of palisade layer and type of stomata. Nevertheless the three Bruguiera species can begrouped anatomically based on the type and pattern of sclereids. Brachy sclereids present in all three taxabut the astero sclereids were found only in
B. gymnorrhiza. Through this anatomical study, we concludedthat all the species have some unique features to withstand extreme environmental habitat.
Keywords: Anatomical characters, anticlinal walls, astero sclereids, Bruguiera, environmental habitat.

29th Kerala Science Congress 27th to 30th January 2017, 09-12
Anatomical, Phytochemical, Morphological and Molecular profiling of blind tree Excoecaria agallocha L. (Euphorbiace)
Surya S and N. Hari

CMS College, Kottayam
Abstract
Excoecaria agallocha L. commonly known as blind tree is a popular mangrove found in mangrove forests. Little is investigated on the morphology, anatomy, and molecular level in this genus, therefore the purpose of this study aiming to explain the anatomy of the blind tree at cellular level, morphological, phytochemical and molecular finger printing. It was explained that many anatomical features of this plant were similar to those of a typical dicotyledonous plant. However, there were showed some unique features because of its salty environmental habitat.

Toluene: Ethyl Acetate: Formic acid: Methanol: 7:5:1:0.5 as good solvent system for measuring the establishment of phytochemicals in HPTLC analysis. It showed Rf values ranged in between 0.10 to 0.80. In evaluation eleven phytochemical compounds present. Molecular markers like rbcL and matK used for clear differentiate Excoecaria in Euphorbiaceae. The nucleotide sequence was also deposited in NCBI.

National seminar on wood product/Technology/Policy & Climatic change.15/02/2017.

A study on leaf anatomy of three mangrove species in relation to climate change
1 S Surya, 2 N Hari

1 Research Scholar, CMS College Kottayam, Kerala, India

2 Assistant Professor, CMS College Kottayam, Kerala, India
Abstract

Mangroves play an irreplaceable role in mitigating the negative effects arising from climate change. Mangrove vegetation helps for reducing greenhouse gases like co2, the main agent in the degradation of ozone layer. Mature leaves of Rhizophora apiculata Blume., Excoecaria agallocha L., and Bruguiera sexangula (Lour.) Poir., were collected from Kollam and Ernakulam districts of Kerala. From each specimen adult leaves were taken from the middle of the crown with a maximum development of leaf. The transverse section were made at a position approximately half way between the base and apex of the lamina and stained with Toluidine blue 0. This study aims to evaluate the anatomical structure of three species in a mangrove established at Kerala state a tool in understanding the spectral responses shown by these species. Individuals of R. apiculata showed higher reflectance values compared to the other species. In E. agallocha the maximum absorptance and transmittance.
Key words: Mangroves, Mitigation, Leaf anatomy, Absorptance, Reflectance, Transmittance.

Journal of Pharmacognosy and Phytochemistry
E-ISSN: 2278-4136, P-ISSN: 2349-8234, JPP 2017; 6(3): 46-50
Received: 23-03-2017, Accepted: 24-04-2017
Preliminary phytochemical study and HPTLC valuation of leaves of grey mangrove Avicennia marina
S Surya Research Scholar, CMS College, Kottayam, Kerala, India
N Hari Assistant Professor, CMS College,Kottayam, Kerala, India

Abstract

Mangroves are the chemical factories of nature and extensive sources of organic chemical matter on thisplanet. The conventional methods are poor, time consuming as well as less systematic, so there is a need to use appear technological knowledge and sophisticated scientific methods to fulfill this knowledge gap.Therefore, present study is an attempt to study the preliminary phytochemistry and distinguish HPTLCpattern of Avicennia marina leaf. To check presence or absence of photochemical compounds likealkaloids, flavonoids, tannins, terpenoids, saponins. reducing sugar, phenols, steroids and glycosideswere identified by preliminary analysis. Toluene: Ethyl Acetate: Formic acid: Methanol: 7:5:1:0.5 as solvent system is the best solvent system for measuring the establishment of phytochemicals of A.marina. HPTLC analysis of methanol extract showed nine peaks and the Rf values ranged in between0.07 to 0.80 at 254nm and Rf values ranged in between 0.07 to 0.81 showing six peaks at 366 nm. It isused as a phytochemical identification tool to check genetic variability in various plant species.
Keywords: Avicennia marina, phytochemicals, HPTLC, extract, leaf.

International Journal of Biology Research
ISSN: 2455-6548, Impact Factor: RJIF 5.22
www.biologyjournal.in
Volume 2; Issue 2; April 2017; Page No. 75-77
Stem anatomical characterstic of rhizophoraceae species in Kerala
*1 S Surya, 2 N Hari

1 Research scholar CMS College Kottayam, Kerala, India

2 Assistant Professor CMS College Kottayam, Kerala, India
Abstract

A study was conducted on the stem anatomical characteristics of six selected Kerala mangrove species, which are representative ofthree genera in Rhizophoraceae. These anatomical features can be very useful in revealing the relationship between the plantspecies and its environment, and the results showed that some anatomical adaptations had occurred in response to extreme climateand exposed environment. These adaptations such as the presence of aerenchyma cells, cutinized epidermal cells and variations inthe presence of parenchyma cells which occurred fairly uniformly among all the species studied.
Keywords: mangroves, anatomy, diffuse porous wood, sclereids.

International Journal of Biology Research
ISSN: 2455-6548, Impact Factor: RJIF 5.22
www.biologyjournal.in
Volume 2; Issue 2; April 2017; Page No. 78-81.
Petiole anatomical characteristics of true mangrove species in Kerala
*1 S Surya, 2 N Hari

1 Research scholar CMS College Kottayam, Kerala, India

2 Assistant Professor CMS College Kottayam, Kerala, India
Abstract

Petiole anatomy of 15 mangrove species in Kerala was investigated to identify and determine its systematic significance that maybe useful in species identification and classification. Variations in petiole anatomical attributes were obvious that they could beused as systematic evidence to taxonomically delineate these taxa even at species level. The use of petiolar anatomical features in systematic description of mangrove species is maiden and innovative and reported for the first time. Findings in this study haveshown some common characters and variations in the petiole anatomical characteristics. Petiole anatomical characteristicsobserved included petiole outlines, patterns of petiole vascular bundles, presence of sclerenchyma cells, presence of phloemsclerenchyma cells, presence of tanniferous and idioblast cells, presence and types of crystals, presence of lignified parenchymacells, and presence and types of trichomes. Results showed that petiole anatomical characteristics can be used as additional data in identification and classification of species in the genus.
Keywords: Petiole, anatomy, mangrove, salt gland, trichome.

International Journal of Pharmaceutical Science and Research
ISSN: 2455-4685, Impact Factor: RJIF 5.28
www.pharmacyjournal.net
Volume 2; Issue 3; May 2017; Page No. 01-06.
IDENTIFICATION OF MEDICINAL MANGROVE ACANTHUS EBRACTEATUS VAHL.,
MORPHOLOGICAL, PHYTOCHEMICAL AND DNA BARCODING METHODS.
1. S Surya, 2. N Hari

1 Research scholar, CMS College Kottayam, Kerala, India

2 Assistant Professor, CMS College Kottayam, Kerala, India.
Abstract

Sea Holly Acanthus ebracteatus Vahl. belongs to Acanthaceae family is a medicinal mangrove plant used as astringent, expectorant, and stimulant. Little is documented on this plant. In this study, aim was to investigate morphological, anatomical, phytochemical and molecular characterstics. Anatomy showed xerophytic characters to withstand extreme environmental conditions. Toluene: Ethyl Acetate: Formic acid: Methanol: 7:5:1:0.5 as solvent system is the best solvent system for measuring the establishment of phytochemicals of A. ebracteatus. HPTLC analysis of methanol extract showed twelve peaks and the Rf values ranged in between 0.07 to 0.85 at 254 nm, eight peaks and Rf values ranged in between 0.07 to 0.85 at 366 nm. The molecular markers rbcL and matK regions clearly distinguished. Moreover, the phylogenetic analysis confirmed their taxonomic position in Acanthaceae. The study denotes DNA barcoding provides successful and precise strategy for increasing the authenticity of species identification. The barcode sequence also was deposited in NCBI. This is the first scientific information about morphology, chemical and DNA fingerprinting of medicinal mangrove A. ebracteatus for medicinal application.
Keywords: acanthaceae, anatomy, phytochemicals, fingerprinting.

International Journal of Research in Pharmacy and
Pharmaceutical Sciences
ISSN: 2455-698X; Impact Factor: RJIF 5.22
www.pharmacyjournal.in
Volume 2; Issue 3; May 2017; Page No. 11-14
Pollen morphology of selected true mangrove species in Kerala
1 S Surya, 2 N Hari

1 Research Scholar, CMS College Kottayam, Kerala, India

2 Assistant Professor, CMS College Kottayam, Kerala, India
Abstract

A study on pollen morphology of fifteen common Indian mangroves from Kerala mangrove swamps. Pollen consists of 9 genera and 15 species belongs to 10 families of true mangrove species has been investigated by light microscope and scanning electron microscope. In present study all the taxa showed few variations based on the characteristics of apertures, ornamentation, size and shape. Through this study gives rise to an idea about pollen type of true mangrove flora in Kerala. Different pollen shapes encountered are Oblate-Spheroidal to Perprolate. Pollen grains were 3- colpate in Acanthus, 3-colporate in Aegiceras, Avicennia, Bruguiera, Excoecaria, Kandelia, Rhizophora and Lumnitzera, 3-pororate in Sonneratia. Exine ornamentation, size and shape of pollen grains are also taken into consider supplementary characters. Present study concluded that most primitive 3-colpate pollen occurs in Acanthaceae; while 3-colporate in five families and the most advanced porate condition in Sonneratiaceae. It appears that aperture evolution has most operated in Acanthaceae followed by Sonneratiaceae.
Keywords: Kerala, palynology, true mangroves, pollen morphology, acetolysis.

International Journal of Pharmaceutical Science and Research
ISSN: 2455-4685, Impact Factor: RJIF 5.28
www.pharmacyjournal.net
Volume 2; Issue 3; May 2017; Page No. 15-19
Leaf and petiole anatomy of some members of
Rhizophoracae (Mangroves) in Kerala
1 S Surya, 2 N Hari

1 Research scholar, CMS College, Kottayam, Kerala, India

2 Assistant Professor, CMS College Kottayam, Kerala, India
Abstract

Leaf and petiole anatomy of Rhizophora apiculata Blume., Rhizophora mucronata Lam., Kandelia candal (L) Druce., were studied. All the species showed unique anatomical features adapted to salty mangrove habitat. Three species showed xerophytic characteristics like presence of thick cuticle, branched sclereids, crystalliferous cells, colourless non- assimilatory water storage tissue in hypodermis, tannin cells and terminal trachieds. Petiole anatomy helps species level identification.
Keywords: mangrove, sclereids, petiole, xerophytes, cuticle.

International Journal of Research in Pharmacy and
Pharmaceutical Sciences
ISSN: 2455-698X; Impact Factor: RJIF 5.22
www.pharmacyjournal.in
Volume 2; Issue 3; May 2017; Page No. 15-17
Studies on preliminary phytochemical analysis of some true mangrove
species in Kerala
1 S Surya, 2 N Hari

1 Research Scholar, CMS College Kottayam, Kerala, India

2 Assistant Professor, CMS College Kottayam, Kerala, India
Abstract

The mangrove plants examined, Acanthus ebracteatus Vahl., Acanthuus ilicifolius Linn., (Acanthaceae), Aegiceras corniculata (L.) Blanco., (Myrsinaceae), Avicennia marina (Forssk.) Vierh., Avicennia officinalis L., (Avicenniaceae), Bruguiera cylindrica (L.) Blume., Bruguiera gymnorrhiza ( L.) Lam., Bruguiera sexangula (Lour.) Poir., Kandelia candel (L.) Druce.Lumnitzera recemosa Willd., (Combretaceae) Rhizophora apiculata Blume.Rhizophora mucronata Lam., (Rhizophoraceae), Excoecaria agallocha L., (Euphorbiaceae), Sonneratia alba Sm., Sonneratia caseolaris (L.) Engl. (Sonneratiaceae). The current study was aimed to have investigation into the active metabolites of phytochemical analysis of the sequential extracts of all species leaves revealed the presence or absence of various bioactive components like alkaloids, flavonoids, tannin, terpenoids, saponins. reducing sugar, phenols, steroids and glycosids.
Keywords: phytochemicals, alkaloids, steroids, Acanthus, Avicennia.

International Journal of Pharmaceutical Science and Research
ISSN: 2455-4685, Impact Factor: RJIF 5.28
www.pharmacyjournal.net
Volume 2; Issue 3; May 2017; Page No. 07-10
Preliminary photochemical assessment and HPTLC characterization of leaves of medicinal mangrove Acanthus ilicifolius L.
1 S Surya, 2 N Hari

1 Research scholar, CMS College Kottayam, Kerala, India

2 Assistant Professor, CMS College Kottayam, Kerala, India
Abstract

Acanthus ilicifolius is a medicinal plant used for treatment of skin diseases, small pox, ulcers, snake bite and rheumatism. The present study investigated preliminary phytochemical estimation and high performance thin layer chromatography (HPTLC) characterization of leaves. Phytochemical compounds like alkaloids, flavonoids, tannin, terpenoids, Saponins, reducing sugar, phenols, steroids and glycosides were identified by preliminary analysis. Toluene: Ethyl Acetate: Formic acid: Methanol: 7:5:1:0.5 as solvent system is the best solvent system for measuring the establishment of photochemical of A. ilicifolius. HPTLC finger print of methanol extract showed eight peaks and the Rf values ranged in between 0.18 to 0.80 at 254nm and in 366 nm it showed seven peaks, Rf values ranged between 0.07 to 0.81. HPTLC analysis helps for the identification of plant species. Further research is necessary to extract the compounds from A. ilicifolius and it will give programmed for further phytochemical and pharmacuetical applications.
Keywords: Acanthus ilicifolius, phytochemicals, tannin, saponin, extract.

International Journal of Pharmaceutical Science and Research
ISSN: 2455-4685, Impact Factor: RJIF 5.28
www.pharmacyjournal.net
Volume 2; Issue 3; May 2017; Page No. 11-14
Leaf anatomical adaptation of some true mangrove species in Kerala
1 S Surya, 2 N Hari

1 Research Scholar, CMS College Kottayam, Kerala, India

2 Assistant Professor, CMS College Kottayam, Kerala, India
Abstract

Mangrove has some unique leaf anatomical features in addition to its morphological characters, which were associated to their adaptation to its saline environments. All the species have thick cuticle and sunken stomata to reduce transpiration. The presence of glandular and non-glandular hairs on the abaxial and adaxial leaf surfaces in some taxa are related to salt secretion of these plants. Numerous water storage tissues occur in the hypodermal or mesophyll tissue of the leaves indicating the modification nature of mangroves in their saline habitat. The presence of terminal tracheids helps with capillary water storage within the leaf. The coriaceous nature of the leaves in some taxa is due to the presence of brachy and astero sclereids with in the mesophyll region.
Keywords: leaf anatomical adaptation true mangrove species morphological characters.

International Journal of Academic Research and Development
ISSN: 2455-4197, Impact Factor: RJIF 5.22
www.academicsjournal.com
Volume 2; Issue 4; July 2017; Page No. 645-648
DNA barcoding as a means for identifying mangrove plants of Kerala
*1 S Surya, 2 N Hari

1 Research Scholar, CMS College, Kottayam, Kerala, India

2 Assistant Professor, CMS College, Kottayam, Kerala, India
Abstract

DNA barcoding is currently gaining popularity due to its simplicity and high accuracy as compared to the complexity and subjective biases associated with morphology-based identification of taxa. The standard chloroplast DNA barcode for land plants recommended by the Consortium for the Barcode of Life (CBOL) plant working group needs to be evaluated for a wide range of plant species. The present study therefore tested the potential of the rbcL and matK marker for the identification of mangrove plants belonging to diverse families of coastal regions. Here, DNA barcoding is being used to identify mangrove plants found in Kerala and distinguished them from other similar species. Several challenges to the successful implementation of plant DNA barcoding are presented and discussed. Despite these challenges, DNA barcoding has the potential to uniquely identify mangrove plants and provide quality control and standardization of the plant material supplied to the pharmaceutical industry.
Keywords: DNA barcoding; rbcL; matK; mangrove plants; identification.

International Journal of Academic Research and Development
ISSN: 2455-4197, Impact Factor: RJIF 5.22
www.academicsjournal.com
Volume 2; Issue 4; July 2017; Page No. 645-648
DNA barcoding as a means for identifying mangrove plants of Kerala
*1 S Surya, 2 N Hari

1 Research Scholar, CMS College, Kottayam, Kerala, India

2 Assistant Professor, CMS College, Kottayam, Kerala, India
Abstract

DNA barcoding is currently gaining popularity due to its simplicity and high accuracy as compared to the complexity and subjective biases associated with morphology-based identification of taxa. The standard chloroplast DNA barcode for land plants recommended by the Consortium for the Barcode of Life (CBOL) plant working group needs to be evaluated for a wide range of plant species. The present study therefore tested the potential of the rbcL and matK marker for the identification of mangrove plants belonging to diverse families of coastal regions. Here, DNA barcoding is being used to identify mangrove plants found in Kerala and distinguished them from other similar species. Several challenges to the successful implementation of plant DNA barcoding are presented and discussed. Despite these challenges, DNA barcoding has the potential to uniquely identify mangrove plants and provide quality control and standardization of the plant material supplied to the pharmaceutical industry.
Keywords: DNA barcoding; rbcL; matK; mangrove plants; identification.

International Journal of Academic Research and Development
ISSN: 2455-4197, Impact Factor: RJIF 5.22
www.academicsjournal.com
Volume 2; Issue 5; September 2017; Page No. 155-158
Evaluation of mangroves from Kerala, west coast India using DNA barcode
*1 S Surya, 2 N Hari

1 Research scholar, CMS College, Kottayam, Kerala, India

2 Assistant Professor, CMS College, Kottayam, Kerala, India
 Abstract

 Mangroves are unique ecosystem exist in tropical and subtropical intertidal zones.  They are most productive, diverse, biologically important ecosystem. Identification of mangrove species is of critical importance in conserving and utilizing biodiversity, which apparently hindered by a lack of taxonomic expertise. In the present study, we performed assessment of four mangrove species of Kerala, west coast India based on core DNA barcode markersrbcL and matK. Present study does not support the species resolution among mangroves using plastid genes such as rbcL and matK. Our future work will be focused on evaluation of other barcode markers to characterize complete resolution of mangrove species and its identification.
Keywords: mangrove, Kerala, DNA barcode, rbcl, matk

International Journal of Scientific Research in Science and Technology
© 2017 IJSRST | Volume 3 | Issue 7 | Print ISSN: 2395-6011 |
Online ISSN: 2395-602X Impact Factor:3.6
Themed Section: Science and Technology
DNA barcoding of Mangroves using Ribosomal ITS Marker in Rhizophoraceae
S. Surya*, N. Hari

CMS College Kottayam, Kottayam, Kerala, India
Abstract

DNA barcode is a very short, standardized DNA sequence in a well-known gene. It provides a way to identify the species to which a plant, animal or fungus it belongs. One of the most popular sequences identification is achieved by comparing DNA sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal cistron. DNA barcoding is a new concept of identifying unknown organisms based on species DNA regions. The nuclear ribosomal Internal Transcribed Spacer region is widely used as a DNA bacoding marker to characterize the diversity and composition of plant groups. The ITS region has been heavily used in both molecular methods and ecological studies, due to its high degree of interspecific variability, conserved primer sites and multiple copy nature in the genome. Primers have long been available for the nuclear ITS (Internal transcribed spacer) rDNA region which are now commonly used for plant identification.
Keywords: Mangrove, Rhizophora, ITS, rDNA.

International Journal of Scientific Research in Science and Technology
© 2017 IJSRST | Volume 3 | Issue 7 | Print ISSN: 2395-6011 |
Online ISSN: 2395-602X Impact Factor:3.6
Themed Section: Science and Technology
Impact of Retted Water (Tank) Treatments on Seed Germination And Vigour Index
In Sesamum indicum L.Var.Kylm-1 And Vigna unguiculata (L.) Walp. Var. Jyothika
C. Priya* and N. Hari

Department of Botany, C.M.S. College, Kottayam, Kerala, India
Abstract

The study was aimed to determine the effects of retted water (Tank) on seed germination and seedling growth of Sesamum indicum L.Var.Kylm-1 and
Vigna unguiculata (L.) Walp. Var. Jyothika plants under laboratory conditions. The effect of retted water were compared to that of zero control (distilled water) and at different concentrations such as 5, 10, 15, 20, 25, 30, 35 ,40 ,45, 50,55,60 and 100% (control) used for seed germination and vigour index of seedlings was studied. It has been concluded that retted water has significantly affected the germination and growth of seeds and seedlings of Sesamum indicum and Vigna unguiculata and that lower concentration was effective for germination and was re-usable for agriculture.
Keywords: Retted water, S. indicum (gingelly), V. unguiculata (cowpea), Seed Germination, Vigour Index

List of NCBI Publications
Research Scholar(Ph.D) and MS.c Students

1. KX231359 Surya S and N. Hari. Lumnitzera racemosa Willd.
voucher SR457-10 ribulose-1,5-bisphosphate carboxylase
/oxygenase large subunit (rbcL) gene, partial cds;
chloroplast. 697 bp linear DNA .24th June 2016.

2. KX231347 Surya S and N. Hari. Lumnitzera racemosa Willd.
voucher SR457-10 maturase K (matK) gene, partial cds;
chloroplast. 854 bp linear DNA. 24th June 2016.

3. KX231362 Surya S and N. Hari. Sonneratia alba Sm.
Voucher SR457-15 ribulose-1,5-bisphosphate
carboxylase/oxygenase large subunit (rbcL) gene,
partial cds; chloroplast. 697 bp linear DNA.
24th June 2016.

4. KX231350 Surya S and N. Hari. Sonneratia alba Sm. voucher
SR457-15 maturase K (matK) gene, partial cds;
chloroplast.863 bp linear DNA.24th June 2016.

5. KX231361 Surya S and N. Hari. Sonneratia caseolaris (L.) Engl.
voucher SR457-14 ribulose-1,5-bisphosphate
carboxylase/oxygenase large subunit (rbcL) gene, partial
cds; chloroplast.697 bp linear DNA.24th June 2016

6. KX231349 Surya S and N. Hari. Sonneratia caseolaris (L.) Engl.
voucher SR457-14 maturase K (matK) gene, partial cds;
chloroplast.863 bp linear DNA.24th June 2016.

7. KX231363 Surya S and N. Hari. Bruguiera cylindrica (L.) Blume.
voucher SR457-6 tRNA-Leu (trnL) gene, partial
sequence; trnL-trnF intergenic spacer, complete sequence;
and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
908 bp linear DNA.24th June 2016.

8. KX463054 Surya S and N. Hari. Bruguiera cylindrica (L.) Blume.
voucher SR457-6 RNA polymerase beta chain (rpoB)
gene, partial cds.508 bp linear DNA.24th June 2016.

9. KX231356 Surya S and N. Hari.. Bruguiera gymnorhiza ( L.) Lam.
voucher SR457-7 ribulose-1,5-bisphosphate
carboxylase/oxygenase large subunit (rbcL) gene, partial
cds; chloroplast.697 bp linear DNA.24th June 2016.

10. KX231344 Surya S and N. Hari. Bruguiera gymnorhiza ( L.) Lam.
voucher SR457-7 maturase K (matK) gene, partial cds;
chloroplast.863 bp linear DNA.24th June
2016.

11. KX231357 Surya S and N. Hari. Bruguiera sexangula(Lour.) Poir.
voucher SR457-8 ribulose-1,5-bisphosphate
carboxylase/oxygenase large subunit (rbcL) gene, partial
cds; chloroplast.697 bp linear DNA.24th June 2016.

12. KX231345 Surya S and N. Hari. Bruguiera sexangula(Lour.) Poir.
voucher SR457-8 maturase K (matK) gene, partial cds;
chloroplast.863 bp linear DNA.24th June 2016.

13. KX231358 Surya S and N. Hari. Kandelia candel (L.) Druce. voucher
SR457-9 ribulose-1,5-bisphosphate
carboxylase/oxygenase large subunit (rbcL) gene, partial
cds; chloroplast.697 bp linear DNA.24th June 2016.

14. KX231346 Surya S and N. Hari. Kandelia candel (L.) Druce. voucher
SR457-9 maturase K (matK) gene, partial cds;
chloroplast.856 bp linear DNA.24th June 2016.

15. KX231364 Surya S and N. Hari. Rhizophora apiculata Blume.
voucher SR457-11 tRNA-Leu (trnL) gene, partial
sequence; trnL-trnF intergenic spacer, complete sequence;
and tRNA-Phe (trnF) gene, partial sequence;
chloroplast.898 bp linear DNA.24th June .2016.

16. KX231337 Surya S and N. Hari. Rhizophora apiculata
Blume.voucher SR457-11 18S ribosomal RNA gene,
partial sequence; internal transcribed spacer 1, 5.8S
ribosomal RNA gene, and internal transcribed spacer 2,
complete sequence; and 28S ribosomal RNA gene, partial
sequence.652 bp linear DNA.24th June 2016.

17. KX231365 Surya S and N. Hari. Rhizophora mucronata Lam.
voucher SR457-12 tRNA-Leu (trnL) gene, partial
sequence; trnL-trnF intergenic spacer, complete sequence;
and tRNA-Phe (trnF) gene, partial sequence;
chloroplast.898 bp linear DNA.24th June 2016.

18. KX231338 Surya S and N. Hari. Rhizophora mucronata Lam.voucher
SR457-12 18S ribosomal RNA gene, partial sequence;
internal transcribed spacer 1, 5.8S ribosomal RNA gene,
and internal transcribed spacer 2, complete sequence; and
28S ribosomal RNA gene, partial sequence.652 bp linear
DNA.24th June 2016.

19. KX231360 Surya S and N. Hari. Excoecaria agallocha L. voucher
SR457-13 ribulose-1,5-bisphosphate
carboxylase/oxygenase large subunit (rbcL) gene, partial
cds; chloroplast.697 bp linear DNA.24th June 2016.

20. KX231348 Surya S and N. Hari. Excoecaria agallocha L. voucher
SR457-13 maturase K (matK) gene, partial cds;
chloroplast.860 bp linear DNA.24th June 2016.

21. KX231353 Surya S and N. Hari. Aegiceras corniculata (L.) Blanco
voucher SR457-3 ribulose-1,5-bisphosphate
carboxylase/oxygenase large subunit (rbcL) gene, partial
cds; chloroplast.697 bp linear DNA.24th June 2016.

22. KX231341 Surya S and N. Hari. Aegiceras corniculata (L.) Blanco
voucher SR457-3 maturase K (matK) gene, partial cds;
chloroplast.860 bp linear DNA.24th June 2016.

23. KX231352 Surya S and N. Hari. Acanthus ebracteatus Vahl., 2
ribulose-1,5-bisphosphate carboxylase / oxygenase large
subunit (rbcL) gene, partial cds; chloroplast. 697 bp linear
DNA .24th June 2016.

24. KX231340 Surya S and N. Hari. Acanthus ebracteatus Vahl.,voucher
SR457-2 maturase K (matK) gene, partial cds;
chloroplast.866 bp linear DNA.24th June 2016.

25. KX231351 Surya S and N. Hari. Acanthuus ilicifolius L., voucher
SR457-1 ribulose-1,5-bisphosphate
carboxylase/oxygenase large subunit (rbcL) gene, partial
cds; chloroplast.697 bp linear DNA.24th June 2016.

26. KX231339 Surya S and N. Hari. Acanthuus ilicifolius L., voucher
SR457-1 maturase K (matK) gene, partial cds;
chloroplast.866 bp linear DNA.24th June 2016.

27. KX231354 Surya S and N. Hari. Avicennia marina (Forssk.) Vierh.
voucher SR457-4 ribulose-1,5-bisphosphate
carboxylase/oxygenase large subunit (rbcL) gene, partial
cds; chloroplast.697 bp linear DNA.24th June 2016.

28. KX231342 Surya S and N. Hari. Avicennia marina (Forssk.) Vierh.
voucher SR457-4 maturase K (matK) gene, partial cds;
chloroplast.866 bp linear DNA.24th June 2016.

29. KX231355 Surya S and N. Hari. Avicennia officinalis L. voucher
SR457-5 ribulose-1,5-bisphosphate
carboxylase/oxygenase large subunit (rbcL) gene, partial
cds; chloroplast.697 bp linear DNA.24th June 2016.

30. KX231343 Surya S and N. Hari. Avicennia officinalis L. voucher
SR457-5 maturase K (matK) gene, partial cds;
chloroplast.866 bp linear DNA.24th June 2016.

31. KX676501 Archana,N.G. and Hari,N. Jasminum multiflorum (Burm.
F.) Andr, voucher SR560A, ribulose-1,5-bisphosphate
carboxylase/oxygenase large subunit (rbcL) gene
partial cds; plastid 666 bp DNA linear.03-OCT-2016

32. KX676500 Archana,N.G. and Hari,N. Jasminum multiflorum (Burm.
F.) Andr, voucher SR560A, maturase K (matK) gene,
partial cds; plastid.798 bp DNA linear. 03-OCT-2016

33. KX676503 Aneesha,K.V. and Hari,N. Jasminum sambac Linn
voucher SR560 B, ribulose-1,5-bisphosphate
carboxylase/oxygenase large subunit (rbcL) gene, partial
cds; plastid.666 bp DNA linear. 03-OCT-2016

34. KX676502 Aneesha,K.V. and Hari,N. Jasminum sambac Linn
voucher SR560 B, maturase K (matK) gene, partial cds;
plastid.. 798 bp DNA linear. 03-OCT-2016

35. KY977984 Sudheeshna,P.K. and Hari,N Jasminum grandiflorum
L.voucher SR627-JG, maturase K (matK) gene,
865 bp DNA linear. 28-AUG-2017

36. KY977985 Sudheeshna,P.K. and Hari,N Jasminum grandiflorum
L.voucher SR627-JG ribulose-1,5-bisphosphate
carboxylase/oxygenase large subunit (rbcL) gene, partial
cds; plastid. 697 bp DNA linear .28-AUG-2017

37. KY977986 Vandana,N.P. and Hari,N.Jasminum azoricum L.
.voucher SR667-JA, RNA polymerase beta subunit(rpoB)
gene, partial cds; plastid. 507 bp DNA linear.28-AUG-
2017

38. KY977987 Vandana,N.P. and Hari,N.Jasminum azoricum L.
.voucher SR667-JA, tRNA-Leu (trnL) gene, partial
sequence; trnL-trnF intergenic spacer, complete sequence;
and tRNA-Phe (trnF) gene, partial sequence; plastid.
857 bp DNA linear .28-AUG-2017